Thermo Scientific™ Tango™ HTR1A-blaU2OS Cells
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1857-Each-of-1 | In Stock | ₹ 9,41,620.00 |
K1857 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,41,620.00
GST (18%): ₹ 1,69,491.60
Total Price: ₹ 11,11,111.60
Accession Number
NM_000524
Cell State
Dividing Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
For Use With (Application)
FRET
Human Protein Class
G-Protein Coupled Receptor (GPCR)
Product Line
Tango™
Readout
End Point
Cell Line
U2OS
Druggable Target
GPCR
Assay Entry
Cell-based beta-arrestin recruitment
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ HTR1A-UAS-bla CHO-K1 cells• Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method
Fluorescence
Gene Symbol
HTR1A
Population Status
Single Cell Clone Origin
Quantity
8 x 106Cells
Target Entry
5HT1A, HTR1A
System Type
Tango™
Related Products
Description
- Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line
- This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element
- Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of 5-CT (Figure 1)
- In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.