Axl Monoclonal Antibody (DS7HAXL), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3
• This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells
• It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis
• Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis
• Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• SB-4400) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma
• The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation
• TAM receptors are also involved in phagocytosis of apoptotic cells
• Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies
• In addition, Axl may function as a putative entry receptor for filoviruses
• Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells
• Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C
• Soluble Axl is generated by proteolytic cleavage of the membrane form
• Increased plasma levels may be indicative of inflammation and cancer.