7068202
FR4 Monoclonal Antibody (eBio12A5), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
FR4
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
0910001L11Rik; C86255; Folate receptor 4; folate receptor 4 (delta); folate receptor 4 (delta) homolog; folate receptor 4, delta (putative); folate receptor delta; Folate-binding protein 3; Folbp3; Folr4; FR-delta; IZUMO1 receptor protein JUNO; IZUMO1 receptor, JUNO; Izumo1r; Juno; probable folate receptor delta; sperm-egg fusion protein Juno
Gene Symbols
Izumo1r
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Izumo1r
Clone
eBio12A5
Applications
Flow Cytometry
Conjugate
FITC
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q9EQF4
Gene ID (Entrez)
64931
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR delta and FBP3 (folate binding protein3)
- FR4 is a heavily glycosylated, 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate
- Natural Treg have high levels of FR4 and together with CD25 can be used to distinguish natural Treg, effector T cells, memory-like T cells and naive T cells
- FR4 high CD25 high cells are natural Treg with high levels of Foxp3
- FR4 high CD25 low cells are identified as central memory T cells, which upon stimulation, can proliferate and produce large amounts of IL-2
- In contrast, FR4 low CD25 high cells secrete proinflammatory cytokines such as IFN gamma and IL-17 and have been characterized as effector memory T cells
- Based on co-staining studies, the epitopes recognized by eBioTH6 and eBio12A5 are different, thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5
- Applications Reported: This eBio12A5 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBio12A5 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate
- Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naive T cells
- In vivo studies with eBioTH6 show a decrease in the number of CD4+CD25+ cells and an enhanced tumor response leading to the elimination of advanced stages of tumors.