MilliporeSigma™ phospho BRD4 (Ser492), Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE1451MI-Each-of-1 | In Stock | ₹ 40,633.84 |
ABE1451MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,633.84
GST (18%): ₹ 7,314.091
Total Price: ₹ 47,947.931
Antigen
phospho BRD4 (Ser492)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
BRD4, HUNK1, MCAP
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
The target phospho-serine residue is present in all human (pSer492) and murine (pSer493) BRD4 spliced isoforms reported by UniProt (O60885 and Q9ESU6). This polyclonal antibody is shown to exhibit stronger immunoreactivity toward BRD4 phosphopeptide with dual pSer492/494 (equivalent to mouse pSer493/495) phosphorylation than the corresponding peptide with only pSer492 (equivalent to mouse Ser493) phosphorylation (Korb, E., et al. (2015). Nat. Neurosci. 18(10):1464-1473).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Immunocytochemistry, Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
O60885
Immunogen
KLH-conjugated linear peptide corresponding to a human BRD4 sequence containing phosphorylated Ser492 (equivalent to mouse Ser493).
Quantity
100 μL
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_490597
Target Species
Human
Form
Purified
Related Products
Description
- Bromodomain-containing protein 4 (UniProt O60885; also known as MCAP, Mitotic chromosome-associated protein, Protein HUNK1) is encoded by the BRD4 (also known as HUNK1, MCAP) gene (Gene ID 23476) in human
- BRD4 is a BET (bromodomain and extra-terminal) protein family member that functions as a chromatin reader by binding acetylated lysines in histones
- BRD4 is an important transcription regulator in many cell types, including transcription in response to external cues
- BRD4 plays an essential role in neuronal function and mediates the transcriptional regulation underlying learning and memory
- BRD4 regulates IEG transcription in neurons upon phosphorylation by casein kinase 2 (CK2), which is activated in response to neuronal stimulation
- Brd4 knockout in mice is lethal, while Brd4 function blockage by inhibitor JQ1 (Cat
- No
- 500586) treatment is reported to affect the transcription of critical synaptic proteins, resulting in memory deficits and decreased seizure susceptibility in mice.