MilliporeSigma™ phospho-Keratin-8 (Ser23), Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABT1372-Each-of-1 | In Stock | ₹ 42,969.20 |
ABT1372 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,969.20
GST (18%): ₹ 7,734.456
Total Price: ₹ 50,703.656
Antigen
phospho-Keratin-8 (Ser23)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
KRT8, CYK8
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This rabbit polyclonal antibody detected the target phospho band only in lysates from phosphatase inhibitors-treated, but not untreated HeLa cells. Antibody blocking by the immunogen peptide, but not the corresponding nonphosphorylated peptide prior to immunoblotting abolished target band detection. The Ser23 designation is based on human keratin-8 sequence with initiator Met1 removed. The phosphorylation site sequence in mouse/rat (-SSR(pS)YT-) differs from that of human (-SSR(pS)FT-) by a single Phe24-to-Tyr24 switch (F24Y). Cross-reactivity of this polyclonal antibody toward murine and rat species is likely, but has not been confirmed.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P05787
Immunogen
KLH-conjugated linear peptide corresponding to the phosphorylation site sequence containing the phosphorylated target serine.
Quantity
100 μL
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_002264
Target Species
Human
Related Products
Description
- Keratin, type II cytoskeletal 8 (UniProt P05787; also known as CK-8, Cytokeratin-8, K8, Keratin-8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID 3856) in human
- Cytokeratin-8 (Keratin-8 or K8) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues
- Each epithelium is characterized by its unique keratin expression pattern
- Keratin 8 is a type II intermediate filament protein found in simple epithelia, where it pairs with keratin 18 (K8/KI8) and plays an important role in protecting cells from stress, injury, and apoptosis
- The structure and function of K8/18 are regulated by posttranslational modifications, including glycosylation, ubiquitination, and phosphorylation
- K8 has been reported to be phosphorylated on Ser8, Ser23, Ser73, and Ser431 by various protein kinases
- PKCepsilon is reported to mediate K8 phosphorylation on Ser8 and Ser23, p38 and PKCdelta are responsible for K8 Ser73 phosphorylation, while ERK and JNK are involved in K8 Ser431 phosphorylation.