GRK1 Monoclonal Antibody (D11), Invitrogen™

Description

• MA1-721 detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues
• This antibody does not cross-react with other G protein-coupled kinases
• MA1-721 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation experiments
• By Western blot, this antibody detects an ∽60 kDa protein representing GRK 1a/b
• MA1-721 detects both in vitro expressed splice variants by Western blot but was not able to detect significant amounts of GRK1b from human retina
• Immunofluorescence staining of GRK1a/b in bovine retina with MA1-721 results in intense staining primarily in cone outer segments, but also in rod outer segments
• Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods
• The MA1-721 immunogen is full length human GRK1a
• This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b
• GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases
• GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation
• Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2)
• GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro
• It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.