MABE948MI
MilliporeSigma™ anti-BRE1-A (RNF20) Clone: 8A4.2,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE948MI-Each-of-1 | In Stock | ₹ 41,813.98 |
MABE948MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,813.98
GST (18%): ₹ 7,526.516
Total Price: ₹ 49,340.496
Antigen
BRE1-A (RNF20)
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
Q5VTR2
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
8A4.2
Formulation
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
RNF20, BRE1, BRE1A
Immunogen
GST-tagged recombinant protein corresponding to the third coiled-coil region of human BRE1-A (RNF20).
Quantity
100 μg
Research Discipline
Epigenetics, Nuclear Function
Gene ID (Entrez)
NP_062538
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG2a κ
Related Products
Description
- Specifically detects BRE1-A (RNF20) Clone: 8A4.2 in Human samples, and it is validated for Western Blotting E3 ubiquitin-protein ligase BRE1A (UniProt Q5VTR2; also known as BRE1 E3 ubiquitin ligase homolog, BRE1-A, hBRE1, Homolog of S
- cerevisiae BRE1, RING finger protein 20) is encoded by the RNF20 (also known as BRE1, BRE1A) gene (Gene ID 56254) in human
- BRE1A (RNF20) is a RING finger domain-containing protein originally identified as the E3 uqibiquitin ligase that catalyzes histone H2B Lys120 monoubiquitination in yeast
- The histone chaperone FACT is reported to mediate RNF20 recruitment for chromatin remodeling to initiate homologous recombination repair (HRR)
- RNF20 is also shown to negatively regulate hepatic fatty acid metabolism by targeting SREBP1c for ubiquitination and subsequent proteasomal degradation
- In addition, RNF20 is shown to mediated the polyubiquitination of ErbB3-binding protein 1 (Ebp1) and Transcription factor activator protein 2α (AP-2α)
- AP-2α functions as a negative regulator of adipogenesis by suppressing the transcription of CCAAT/enhancer binding protein (C/EBPα)
- Overexpression of RNF20 in 3T3-L1 cells results in a down-regulation of endogenous AP-2α and a concomitant transcriptional upregulation of C/EBPα.