MABE967MI
MilliporeSigma™ anti-DNA polymerase delta p125 Clone: 11E10.1,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE967MI-Each-of-1 | In Stock | ₹ 44,393.20 |
MABE967MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,393.20
GST (18%): ₹ 7,990.776
Total Price: ₹ 52,383.976
Antigen
DNA polymerase delta p125
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
P28340
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
11E10.1
Formulation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
POLD1, POLD
Immunogen
GST-tagged recombinant protein corresponding to the N-terminus of human DNA polymerase delta p125.
Quantity
100 μg
Research Discipline
Epigenetics, Nuclear Function
Gene ID (Entrez)
NP_001243778
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG2b κ
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Description
- Specifically detects DNA polymerase delta p125 Clone: 11E10.1 in Human samples, and it is validated for Western Blotting DNA polymerase delta catalytic subunit (EC 2.7.7.7; UniProt P28340; also known as CDC2 homolog, DNA polymerase subunit delta p125) is encoded by the POLD1 (also known as CDC2, MDPL, POLD, CRCS10) gene (Gene ID 5424) in human
- DNA replication in eukaryotes involves multiple enzymes including the B family polymerases Pol α, Pol δ, and Pol ?
- While Pol α initiates DNA synthesis, Pol δ and Pol ? replace Pol α after primer extension and perform the bulk of DNA replication
- Pol δ and Pol ? are heterotetramers in higher eukaryotes comprised of POLD1 and POLE as the respective catalytic subunit, as well as accessory subunits (POLD2/3/4 and POLE2/3/4) that interact with cofactors such as Proliferating Cell Nuclear Antigen (PCNA)
- Both POLD1 and POLE contain an exonuclease domain (a.a
- 268-471 of POLE and 304-517 of POLD1) that proof-reading capability during DNA replication
- In addition to DNA synthesis, Pol ? and Pol δ are believed to participate in chromosomal DNA repairs, including nucleotide excision repair (NER), ismatch repair (MMR) and repair of double strand breaks (DSBR)
- Germline exonuclease domain mutations (EDMs) in POLD1 and POLE cause polymerase proofreading-associated polyposis (PPAP)
- Tumors with EDMs exhibits dramatically increased base substitutions.