Phospho-SMAD2 (Ser250) Recombinant Rabbit Monoclonal Antibody (SD207-1), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA532359-Each-of-1 | In Stock | ₹ 46,413.50 |
PIMA532359 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 46,413.50
GST (18%): ₹ 8,354.43
Total Price: ₹ 54,767.93
Antigen
Phospho-SMAD2 (Ser250)
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide, pH 7.4
Gene Accession No.
O70436, Q15796, Q62432
Gene Symbols
SMAD2
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser250 of Human Smad2 aa 221-270
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Applications
Flow Cytometry, Western Blot
Clone
SD207-1
Conjugate
Unconjugated
Gene
SMAD2
Gene Alias
7120426M23Rik, EGK_09664, hMAD-2, hSMAD2, JV18, JV18-1, MAD, MAD homolog 2, Mad protein homolog, Madh2, Madr2, Mad-related protein 2, MGC22139, MGC34440, mMad2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against decapentaplegic-like protein 2, mothers against DPP homolog 2, pSMAD2, Sma- and Mad-related protein 2, SMAD, SMAD 2, SMAD family member 2, SMAD, mothers against DPP homolog 2, Smad2, Smad-2, Smad2Deltaexon3
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
17126, 29357, 4087
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Related Products
Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation
- SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling
- Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin
- In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors
- The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4
- The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors
- SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin
- Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.