Phospho-N-WASP (Tyr256) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143792-Each-of-1 | In Stock | ₹ 45,879.50 |
PIPA5143792 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,879.50
GST (18%): ₹ 8,258.31
Total Price: ₹ 54,137.81
Antigen
Phospho-N-WASP (Tyr256)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
WASL
Gene Alias
2900021I12Rik; 3110031I02Rik; DKFZp779G0847; MGC48327; n wasp; neural Wiskott-Aldrich syndrome protein; NWASP; N-WASP; TRS4; WASL; WASP like actin nucleation promoting factor; WASPB; Wiskott-Aldrich syndrome like; Wiskott-Aldrich syndrome-like; Wiskott-Aldrich syndrome-like (human)
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
682507, 73178, 8976
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Applications
Immunocytochemistry, Western Blot
Concentration
Lot-Specific
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
O00401, O08816, Q91YD9
Gene Symbols
WASL
Immunogen
Phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
Related Products
Description
- This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier)
- The antibody detects a 65 kDa* protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate
- A similar band is observed in pervanadate treated HeLa and endothelial cells
- Weak bands are also observed at higher molecular weights after pervanadate treatment
- These bands may be due to low cross-reactivity with phosphotyrosine
- The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton
- The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins
- Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3
- The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues
- It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.