Phospho-WAVE1 (Tyr125) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143794-Each-of-1 | In Stock | ₹ 45,879.50 |
PIPA5143794 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,879.50
GST (18%): ₹ 8,258.31
Total Price: ₹ 54,137.81
Antigen
Phospho-WAVE1 (Tyr125)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
WASF1
Gene Alias
AI195380; AI838537; homology of dictyostelium scar 1; KIAA0269; Protein WAVE-1; Scar; SCAR1; Verprolin homology domain-containing protein 1; WAS protein family; WAS protein family member 1; WAS protein family, member 1; WASF1; WASP family 1; WASP family protein member 1; WAVE; WAVE1; WAVE-1; Wiskott-Aldrich syndrome protein family member 1
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
294568, 83767, 8936
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Applications
Immunocytochemistry, Western Blot
Concentration
Lot-Specific
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
Q5BJU7, Q8R5H6, Q92558
Gene Symbols
WASF1
Immunogen
Phospho-WAVE (Tyr-125) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-125 in human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in the conserved site in WAVE2 (Tyr-124) and WAVE3 (Tyr-125).
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
Related Products
Description
- This antibody was cross-adsorbed to phospho-WAVE (Tyr-150) and unphosphorylated WAVE (Tyr-125) peptides before affinity purification using phospho-WAVE (Tyr-125) peptide
- In western blots, the antibody detects an 80 kDa* band corresponding to phosphorylated WAVE in human SYF cSrc transformed cells and mouse macrophages treated with pervanadate, but is not observed in control cells
- These bands can be specifically blocked with phospho-WAVE (Tyr-125) peptide (WX1775), but not phospho-WAVE (Tyr-150) peptide (WX1825)
- WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization
- The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton
- The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases
- The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac
- The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments
- WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes
- WAVE3 forms a multiprotein complex that links receptor kinases and actin
- Binding to actin occurs through a C-terminal verprolin homology domain in all family members
- The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function.