MABN1182MI
MilliporeSigma™ Glutamine Synthetase, Mouse, Unlabeled, Clone: 11A3.1,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABN1182MI-Each-of-1 | In Stock | ₹ 42,968.31 |
MABN1182MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,968.31
GST (18%): ₹ 7,734.296
Total Price: ₹ 50,702.606
Antigen
Glutamine Synthetase
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P09606
Immunogen
GST-tagged recombinant rat glutamine synthetase internal fragment.
Quantity
100 μL
Research Discipline
Neuroscience
Gene ID (Entrez)
NP_058769
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
11A3.1
Conjugate
Unconjugated
Gene
Glul, Glns
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 11A3.1 targets an internal epitope within the C-terminal half of human and rat glutamine synthetase.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2a κ
Related Products
Description
- Glutamine synthetase (EC 4.1.1.15, EC 6.3.1.2; UniProt P09606; also known as Glutamate-ammonia ligase, Glutamate decarboxylase, GS) is encoded by the Glul (also known as Glns) gene (Gene ID 24957) in rat species
- Glutamine synthetase (GS) plays an essential role in nitrogen metabolism by catalyzing ATP-dependent condensation of glutamate and ammonia to form glutamine
- Three classes of GS exist, class I enzymes (GSI) are specific to prokaryotes and exist as oligomers of 12 identical subunits, class II enzymes (GSII) exist as decamer of identical subunits in eukaryotes and in some bacteria, while class III enzymes (GSIII) function as a double-ringed dodecamer of identical chains found in bacteroides fragilis and in butyrivibrio fibrisolvens
- GS is present predominantly in the brain, kidneys, and liver in mammals
- In the brain, GS is found primarily in astrocytes, where it participates in the the detoxification of ammonia via assimilation and termination of neurotransmitter signals via recyclization
- c-Myc promotes glutamine usage by upregulating glutaminase (GLS), which converts glutamine to glutamate in the TCA cycle, while in some cancer cells Myc-mediated thymine DNA glycosylase (TDG) transcription leads to GS upregulation due to TDG-dependent demethylation of the GS promoter
- GS upregulation promotes cancer cell survival under glutamine limitation, while GS silencing decreases cancer cell proliferation and xenograft tumor growth.