MABN1537MI
MilliporeSigma™ TDO2, Mouse, Unlabeled, Clone: 7A6.1,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABN1537MI-Each-of-1 | In Stock | ₹ 40,633.84 |
MABN1537MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,633.84
GST (18%): ₹ 7,314.091
Total Price: ₹ 47,947.931
Antigen
TDO2
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P48775
Immunogen
GST-tagged recombinant human TDO2 internal fragment.
Quantity
200 μL
Research Discipline
Neuroscience
Gene ID (Entrez)
NP_005642
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
7A6.1
Conjugate
Unconjugated
Gene
TDO2, TDO
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 7A6.1 targets an internal epitope of human and murine TDO2 (TDO).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2a κ
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Description
- E3 ubiquitin-protein ligase RNF43 (UniProt Q68DV7; also known as RING finger protein 43) is encoded by the RNF43 gene (Gene ID 54894) in human
- RNF43 and the homologous ZNRF3 are cell-surface transmembrane E3 ubiquitin ligases upregulated upon Wnt/ -catenin signaling and act as potent Wnt signaling pathway negative feedback regulators by mediating ubiquitination of Frizzled (FZD) and LRP6 for lysosomal degradation
- RNF43/ZNRF3 activity is counteracted by stem cell growth factor R-spondin that induces membrane ZNRF3/RNF43 clearance by binding ZNRF3/RNF43 and LGR4/LGR5
- Physical interaction with Dishevelled (DVL) is essential for ZNRF3/RNF43 Wnt inhibitory activity, and DVL knockdown significantly increases cell surface levels of FZD and LRP6
- In addition, RNF43 suppresses canonical and noncanonical Wnt signaling via distinct mechanisms
- While interaction between the extracellular protease-associated (PA) domain and the cysteine-rich domain (CRD) of FZD and RNF43 intracellular RING finger domain is necessary for canonical Wnt/ -catenin signaling inhibition, interaction between FZD PDZ domain and RNF43 C-terminal cytoplasmic region is essential for suppressing noncanonical Wnt signaling
- R-spondin overexpression through gene translocation and loss-of-function RNF43/ZNRF3 gene mutations are found in human cancers as means to escape from Wnt signaling negative feedback regulation
- Human RNF43 is produced with a signal peptide (a.a
- 1-23) sequence, which is posttranslationally removed to yiled the mature enzyme with an N-terminal extracellular domain (a.a
- 24-197), a transmembrane domain (a.a
- 198-218), and a large cytoplasmic region (a.a
- 219-783) that contains an atypical RING-type zinc finger (a.a
- 272-313) and a pro-rich (a.a
- 569-760) region.