CD11a/CD18 Monoclonal Antibody (24), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA533356-Each-of-1 | In Stock | ₹ 98,434.00 |
PIMA533356 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 98,434.00
GST (18%): ₹ 17,718.12
Total Price: ₹ 1,16,152.12
Antigen
CD11a/CD18
Classification
Monoclonal
Concentration
100 μg/mL
Formulation
PBS with 0.1% BSA and no preservative
Gene Accession No.
P05107, P20701
Gene Symbols
ITGAL, ITGB2
Purification Method
Protein G
Regulatory Status
RUO
Gene ID (Entrez)
3683, 3689
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation
Clone
24
Conjugate
Unconjugated
Gene
ITGAL
Gene Alias
antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11a; CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin alpha L; integrin alpha-L; integrin beta chain, beta 2; integrin beta-2; integrin gene promoter; integrin subunit alpha L; integrin subunit beta 2; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGAL; ITGB2; LAD; LCAMB; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte cell adhesion molecule CD18; leukocyte function-associated molecule 1 alpha chain; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; MAC-1; MF17; MFI7
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1
Related Products
Description
- Product is supplied at 1 mL (100 μL/mL)
- This antibody inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2-activated natural killer cell assays
- The antibody does not interfere with mitogen-stimulated T cell proliferation
- Furthermore it prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation
- Application Notes: Flow cytometry - This antibody stains the extracellular domain of CD11/CD18
- Functional Assay -This antibody acts as an inhibitor
- The antibody was functionally tested by inhibition of antigen-specific T cell proliferation and LAK cell cytotoxy, which are LEA-1-dependent, and f-Met-Leu-Phe-stimulated chemotaxis, which is a CR3-dependent process
- Positive control: PMN, monocytes, lymphocytes
- CD11/CD18 (LFA-1) are beta 2-type integrins
- The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions
- T cells use integrins in essentially all of their functions
- Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering
- The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg2+
- The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to the leukocyte integrins and thus reflects functionally active molecules
- The epitope can be induced on polymorphonuclear leukocytes and on monocytes
- Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding
- The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays
- The antibody does not interfere with mitogen-stimulated T cell proliferation
- Furthermore the monoclonal antibody 24 prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation.